Progesterone Receptor (PGR) Mouse Monoclonal Antibody [Clone ID: UMAB135]

CAT#: UM800036CF

Carrier-free (BSA/glycerol-free) PR (PgR) mouse monoclonal antibody,clone UMAB135

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGR (RC221303, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGR (1:500).

• 

  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

• 

  Western blot analysis of extracts (35ug) from T47D cells by using anti-PGR monoclonal antibody (1:500).

• 

  Western blot of human tissue lysates (15ug) from 8 different tissues (1: Uterus, 2: Breast, 3: Brain, 4: Liver, 5: Ovary, 6: Thyroid, 7: Colon, 8: Spleen ). Diluation: 1:500.

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PGR mouse monoclonal antibody. (UM800036; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGRPGRmonoclonal antibody. (UM800036; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded normal/normal adjacent breast with mouse anti-Progesterone [PgR] clone UMAB135 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of progesterone is nuclear in normal/normal adjacent breast tissue.

• Immunohistochemical staining of paraffin-embedded normal/normal adjacent breast with mouse anti-Progesterone [PgR] clone UMAB135 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of progesterone is nuclear in normal/normal adjacent breast tissue.

• Immunofluorescent staining of MCF-7 cells using anti-PGR mouse monoclonal antibody (UM800036, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

• Immunofluorescent staining of T47D cells using anti-PGR mouse monoclonal antibody (UM800036, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

• HEK293T cells transfected with either RC221303 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PGR antibody (UM800036), and then analyzed by flow cytometry (1:100).

Show all 11 images

Specifications

Product Data

• Clone Name: UMAB135
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-298 of human PGR (NP_000917) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens progesterone receptor (PGR), transcript variant 2, mRNA.
• Database Link:
  NP_000917
  Entrez Gene 5241 Human
  UniProt ID P06401
• Background: This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]
• Synonyms: NR3C3; PR

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation