CD146 (MCAM) Mouse Monoclonal Antibody [Clone ID: UMAB155]

CAT#: UM800052CF

Carrier-free (BSA/glycerol-free) CD146 (MCAM) mouse monoclonal antibody,clone UMAB155

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

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  Western blot analysis of HEK293 cell lysate (35ug) by using anti-MCAM monoclonal antibody.

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  Western blot analysis of Hela cell lysate (35ug) by using anti-MCAM monoclonal antibody.

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  Western blot analysis of MALME3M cell lysate (35ug) by using anti-MCAM monoclonal antibody.

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MCAMmouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human melanoma using anti-CD146 (MCAM) clone UMAB155 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800052 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110°C for 3min using pressure chamber/cooker. The tumor cells shows strong membraneous and cytoplasmic staining.

• Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD146 clone UMAB155 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800052) (1:300).

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Specifications

Product Data

• Clone Name: UMAB155
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCAM (NP_006491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens melanoma cell adhesion molecule (MCAM), mRNA.
• Database Link:
  NP_006491
  Entrez Gene 4162 Human
  UniProt ID P43121
• Synonyms: CD146; MUC18

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane