Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: UMAB159]

CAT#: UM800054CF

Carrier-free (BSA/glycerol-free) Vimentin (VIM) mouse monoclonal antibody,clone UMAB159

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM (1:500).

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  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and VIM-Knockout Hela cells (KO, Cat# LC810257) were separated by SDS-PAGE and immunoblotted with anti-VIM monoclonal antibody UM800054, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VIM mouse monoclonal antibody. (UM800054; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min) (1:200)

• Immunohistochemical staining of paraffin-embedded human glioma using anti-vimentin clone UMAB159 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800054 requires HIER with with Accel 3in1 EDTA solution pH8.7 at 110°C for 3min using pressure chamber/cooker. The neural cells shows membrane and cytoplasmic staining.

• Immunohistochemical staining of paraffin-embedded human tonsil using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer usin

• Immunohistochemical staining of paraffin-embedded human three cases of gastric cancer using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate

• Immunohistochemical staining of two case of paraffin-embedded human ovarain cancer using anti-Vimentin clone UMAB159 mouse mAb (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer

• Immunohistochemical staining of paraffin-embedded human placenta using anti-Vimentin clone UMAB159 mouse monoclonal antibody (UM800054) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer us

• Immunofluorescent staining of HeLa cells using anti-Vimentin mouse monoclonal antibody (UM800054, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Show all 21 images

Specifications

Product Data

• Clone Name: UMAB159
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens vimentin (VIM), mRNA.
• Database Link:
  NP_003371
  Entrez Gene 22352 MouseEntrez Gene 81818 RatEntrez Gene 7431 Human
  UniProt ID P08670
• Background: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009]
• Synonyms: CTRCT30; HEL113

Reference Data

• Protein Families: ES Cell Differentiation/IPS