Hemogen (HEMGN) Mouse Monoclonal Antibody [Clone ID: UMAB169]

CAT#: UM800062CF

Carrier-free (BSA/glycerol-free) HEMGN mouse monoclonal antibody,clone UMAB169

Product Images

Western blot of cell lysates (35ug) from HL-60. Diluation: 1:500

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  Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-HEMGN Mouse monoclonal antibody. (UM800062; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-HEMGN mouse monoclonal antibody (UM800062). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Specifications

Product Data

• Clone Name: UMAB169
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 260-484 of human HEMGN (NP_060907) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.2 kDa
• Gene Name: Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.
• Database Link:
  NP_060907
  Entrez Gene 55363 Human
  UniProt ID Q9BXL5
• Synonyms: CT155; EDAG; EDAG-1; NDR