MLH1 Mouse Monoclonal Antibody [Clone ID: UMAB191]

CAT#: UM800083CF

Carrier-free (BSA/glycerol-free) MLH1 mouse monoclonal antibody,clone UMAB191

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

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  Western blot of mouse tissue lysates (20ug) from Uterus. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800083) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800083) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800083) (1:200)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800083) (1:200)

• Immunofluorescent staining of HeLa cells using anti-MLH1 mouse monoclonal antibody (UM800083, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB191
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1(NP_000240) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.4 kDa
• Gene Name: Homo sapiens mutL homolog 1 (MLH1), transcript variant 1, mRNA.
• Database Link:
  NP_000240
  Entrez Gene 17350 MouseEntrez Gene 81685 RatEntrez Gene 4292 Human
  UniProt ID P40692
• Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009]
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer