CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: UMAB192]

CAT#: UM800084CF

Carrier-free (BSA/glycerol-free) NCAM1/CD56 mouse monoclonal antibody,clone UMAB192

Product Images

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800084) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-NCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800084) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800084) (1:200)

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NCAM1 mouse monoclonal antibody (UM800084). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

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Specifications

Product Data

• Clone Name: UMAB192
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 94.4 kDa
• Gene Name: Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mRNA.
• Database Link:
  NP_851996
  Entrez Gene 17967 MouseEntrez Gene 24586 RatEntrez Gene 4684 Human
  UniProt ID P13591
• Background: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
• Synonyms: CD56; MSK39; NCAM

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Prion diseases