TNFRSF18 Mouse Monoclonal Antibody [Clone ID: UMAB203]

CAT#: UM800095CF

Carrier-free (BSA/glycerol-free) TNFRSF18/CD357 mouse monoclonal antibody,clone UMAB203

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TNFRSF18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800095) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-TNFRSF18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800095) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TNFRSF18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800095) (1:200)

• 

  Immunofluorescent staining of HeLa cells using anti-TNFRSF18 mouse monoclonal antibody (UM800095, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• 

  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNFRSF18 mouse monoclonal antibody (UM800095). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Show all 5 images

Specifications

Product Data

• Clone Name: UMAB203
• Applications: 10k-ChIP, IF, IHC
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-162 of human TNFRSF18(NP_004186) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 18 (TNFRSF18), transcript variant 1, mRNA.
• Database Link:
  NP_004186
  Entrez Gene 8784 Human
  UniProt ID Q9Y5U5
• Background: This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb
• Synonyms: AITR; CD357; GITR; GITR-D

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction