LGR5 Mouse Monoclonal Antibody [Clone ID: UMAB212]

CAT#: UM800104CF

Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody,clone UMAB212

Product Images

NIH-3T3 (Left lane) or stable expressed LGR5-3T3 (RC212825, Right lane) cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5 (1:1000).

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  Western blot analysis of extracts (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody (1:2000). (Lanes: control vector pCMV6-Entry (PS100001); human LGR4 (RC221345), LGR5 (RC212825) and LGR6 (RC219284); or mouse LGR4 (MR219497), LGR5 (MR219702) and LGR6 (MR224646)).

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  Western blot analysis of extracts (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody (left lane: Brain right lane: Testis) (1:2000).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800104) (1:150)

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  Immunohistochemical staining of paraffin-embedded human small intestine using anti-LGR5 clone UMAB212 mouse monoclonal antibody used at 1ug/mL dilution and detection with Polink2 Broad HRP DAB. UM800104 requires heat-induced epitope retrieval with TEE pH 9.0 at 95-100C 20 minutes. The image shows two arrows pointing to strong and weak staining of small intestine stem cells.

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  Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 clone UMAB212 (UM800104) at 1:400 with GBI Polink2 Broad HRP DAB detection kit (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 (B21-100)using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.

• Immunohistochemical staining of paraffin-embedded human gastric cancer using mouse anti-LGR5 clone UMAB212 (UM800104) at 1:400 with GBI Polink2 Broad HRP DAB detection kit (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 (B21-100) using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.

• Anti-LGR5 mouse monoclonal antibody (UM800104)immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY human LGR5 (RC212825) (1:100).

• Anti-LGR5 mouse monoclonal antibody (UM800104) immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5 (MR219702) (1:100).

• Flow cytometric analysis of HEK293T cells transiently transfected with human LGR4 (RC221345) or mouse LGR4 (MR219497), human LGR5 (RC212825) or mouse LGR5 (MR219702), human LGR6 (RC219284) or mouse LGR6 (MR224646), or control vector pCMV6-Entry (PS100001), using anti-LGR5 antibody (UM800104, 1:100) surface stained on live cells.

• Flow cytometric analysis of stable expression LGR5 (RC212825) HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (UM800104, 1:100) surface stained on live cells.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGR5 mouse monoclonal antibody (UM800104) at 1:100. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB212
• Applications: 10k-ChIP, FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:100~150 (recommended antigen retrieval buffer: TEE buffer pH 9.0), IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 99.8 kDa
• Gene Name: Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.
• Database Link:
  NP_003658
  Entrez Gene 14160 MouseEntrez Gene 299802 RatEntrez Gene 8549 Human
  UniProt ID O75473
• Synonyms: FEX; GPR49; GPR67; GRP49; HG38

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane