c Kit (KIT) Mouse Monoclonal Antibody [Clone ID: UMAB216]

CAT#: UM800108CF

Carrier-free (BSA/glycerol-free) KIT mouse monoclonal antibody,clone UMAB216

Product Images

Immunohistochemical staining of paraffin-embedded human gastric carcinoma using anti-KIT mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800108 was diluted 1:400 and detection was done with HRP secondary and DAB chromogen. Cytoplasmic and mebraneous positive stain seen in very few tumor cells.

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  Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-KIT mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800108 was diluted 1:400 and detection was done with HRP secondary and DAB chromogen. Cytoplasmic and mebraneous stain seen in the tumor cells.

Specifications

Product Data

• Clone Name: UMAB216
• Applications: IHC
• Recommended Dilution: IHC 1:400
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-308 of human KIT(NP_000213) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 109.7 kDa
• Gene Name: Homo sapiens KIT proto-oncogene, receptor tyrosine kinase (KIT), transcript variant 1, mRNA.
• Database Link:
  NP_000213
  Entrez Gene 3815 Human
  UniProt ID P10721
• Background: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: C-Kit; CD117; PBT; SCFR

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer