PD L1 (CD274) Mouse Monoclonal Antibody [Clone ID: UMAB229]

CAT#: UM800121CF

Carrier-free (BSA/glycerol-free) PD-L1/CD274 mouse monoclonal antibody, clone UMAB229

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500).

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  Western blot analysis of extracts (35ug) from 10 different cell lines by using anti-SDHB monoclonal antibody. 1:500

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  Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) human tonsil tissue using anti-PD-L1 antibodies from 4 companies. (Heat-induced epitope retrieval by Retrieval Solution (20x)) – Data from Dr. Willa Shi, University Health Network.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121) (1:25)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121) (1:25)

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  Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121) (1:25)

• Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121) (1:25)

• Immunohistochemical staining of paraffin-embedded SK-MEL-28 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800121) (1:25)

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Specifications

Product Data

• Clone Name: UMAB229
• Applications: IHC, WB
• Recommended Dilution: IHC 1:25
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-290 of human CD274 (NP_054862) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.28 kDa
• Gene Name: Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
• Database Link:
  NP_054862
  Entrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)