Villin (VIL1) Mouse Monoclonal Antibody [Clone ID: UMAB230]

CAT#: UM800122CF

Carrier-free (BSA/glycerol-free) Villin (VIL1) mouse monoclonal antibody, clone UMAB230

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-VIL1 (Villin) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800122) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VIL1 (Villin) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800122) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIL1 (Villin) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800122) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-VIL1 (Villin) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800122) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-VIL1 (Villin) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800122) (1:1000)

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  Immunohistochemical staining of paraffin-embedded HT-29 cell pellets using anti-VIL1 (Villin) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800122) (1:1000)

• Immunohistochemical staining of paraffin-embedded LoVo cell pellets using anti-VIL1 (Villin) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800128) (1:1000)

• Immunohistochemical staining of paraffin-embedded Raji cell pellets using anti-VIL1 (Villin) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800122) (1:1000)

• Immunohistochemical staining of paraffin-embedded Huh7 cell pellets using anti-VIL1 (Villin) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800128) (1:1000)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-VIL1 (Villin) mouse monoclonal antibody (UM800122). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100)

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Specifications

Product Data

• Clone Name: UMAB230
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100~1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-188 of human VIL1 (Villin) (NP_009058) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92.5 kDa
• Gene Name: Homo sapiens villin 1 (VIL1), mRNA.
• Database Link:
  NP_009058
  Entrez Gene 22349 MouseEntrez Gene 316521 RatEntrez Gene 7429 Human
  UniProt ID P09327
• Synonyms: D2S1471; VIL