DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: UMAB231]

CAT#: UM800123CF

Carrier-free (BSA/glycerol-free) CD74 mouse monoclonal antibody,clone UMAB231

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74 (RC212964, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD74 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 2.5 min, UM800123) (1:1600)

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  Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

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  Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

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  Immunohistochemical staining of paraffin-embedded HL-60 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

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  Immunohistochemical staining of paraffin-embedded Ramos cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

• Immunohistochemical staining of paraffin-embedded LoVo cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

• Immunohistochemical staining of paraffin-embedded Raji cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800123) (1:1600)

• Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD74 clone UMAB231 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800123) (1:800).

• Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-CD74 clone clone UMAB231 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800123) (1:800).

• Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-CD74 clone UMAB231 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800123) (1:800).

• Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD74 clone UMAB231 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800123) (1:800)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD74 mouse monoclonal antibody (UM800123). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100)

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Specifications

Product Data

• Clone Name: UMAB231
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~1600
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD74(NP_001020330) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.3 kDa
• Gene Name: Homo sapiens CD74 molecule (CD74), transcript variant 1, mRNA.
• Database Link:
  NP_001020330
  Entrez Gene 972 Human
  UniProt ID P04233
• Synonyms: DHLAG; HLADG; Ia-GAMMA; II

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation