DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: UMAB232]

CAT#: UM800124CF

Carrier-free (BSA/glycerol-free) CD74 mouse monoclonal antibody,clone UMAB232

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD74 (RC212964, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD74 (1:500).

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  Western blot analysis of extracts (35ug) from 4 different cell lines and human spleen tissue by using anti-CD74 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded HL-60 cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded LoVo cell pellets using anti-CD74 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800124) (1:800)

• Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue within the normal limits using anti-CD74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA507339) (1:400)

• Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD74 clone UMAB232 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800124) (1:400).

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Specifications

Product Data

• Clone Name: UMAB232
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:800
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD74(NP_001020330) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.3 kDa
• Gene Name: Homo sapiens CD74 molecule (CD74), transcript variant 1, mRNA.
• Database Link:
  NP_001020330
  Entrez Gene 972 Human
  UniProt ID P04233
• Synonyms: DHLAG; HLADG; Ia-GAMMA; II

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation