CD10 (MME) Mouse Monoclonal Antibody [Clone ID: UMAB235]

CAT#: UM800127CF

Carrier-free (BSA/glycerol-free) MME/CD10 mouse monoclonal antibody, clone UMAB235

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MME (RC223013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (1:500).

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  Western blot analysis of extracts (35ug) from 5 different cancer cell lines by using anti-MME (CD10) monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 2 tissues lysates by using anti-MME monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue (On the left is brukitt, on the right is follicular lymphoma) using anti-MME (CD10) Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded NCI-H1650 cell pellets using anti-MME (CD10) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded MDA-MB-453 cell pellets using anti-MME (CD10) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded SK-MEL-28 cell pellets using anti-MME (CD10) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded Ramos cell pellets using anti-MME (CD10) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded Raji cell pellets using anti-MME (CD10) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800127) (1:600)

• Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-MME (CD10) mouse clone UMAB235 monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse bladder tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse testicle tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse embryonic tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Immunohistochemical staining of paraffin-embedded mouse placenta tissue using anti-MME (CD10) clone UMAB235 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800127) (1:300).

• Flow cytometric analysis of living 293T cells transfected with MME overexpression plasmid (RC223013), Red)/empty vector (PS100001, Blue) using anti-MME antibody (UM800127). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: UMAB235
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:600
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 411-750 of human MME (CD10) (NP_009219) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.3 kDa
• Gene Name: Homo sapiens membrane metalloendopeptidase (MME), transcript variant 2a, mRNA.
• Database Link:
  NP_009219
  Entrez Gene 17380 MouseEntrez Gene 24590 RatEntrez Gene 4311 Human
  UniProt ID P08473
• Synonyms: CALLA; CD10; CMT2T; NEP; SCA43; SFE

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Hematopoietic cell lineage, Renin-angiotensin system