alpha smooth muscle Actin (ACTA2) Mouse Monoclonal Antibody [Clone ID: UMAB237]

CAT#: UM800129CF

Carrier-free (BSA/glycerol-free) ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody,clone UMAB237

Product Images

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Human uterine smooth muscle tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded Human uterine leiomyoma tissue using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200).

• Immunohistochemical staining of paraffin-embedded A-431 cell pellets using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800129) (1:3200)

• Immunohistochemical staining of paraffin-embedded NIH/3T3 cell pellets using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800129) (1:3200)

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Specifications

Product Data

• Clone Name: UMAB237
• Applications: IHC
• Recommended Dilution: IHC 1:3200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the N terminal of human alpha smooth muscle actin conjugated to KLH.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.8 kDa
• Gene Name: Homo sapiens actin alpha 2, smooth muscle (ACTA2), transcript variant 2, mRNA.
• Database Link:
  NP_001604
  Entrez Gene 11475 MouseEntrez Gene 81633 RatEntrez Gene 59 Human
  UniProt ID P62736
• Synonyms: AAT6; ACTSA; MYMY5

Reference Data

• Protein Pathways: Vascular smooth muscle contraction