MCM2 Mouse Monoclonal Antibody [Clone ID: UMAB238]

CAT#: UM800130CF

Carrier-free (BSA/glycerol-free) MCM2 mouse monoclonal antibody,clone UMAB238

Product Images

Western blot analysis of extracts (35ug) from 5 different cancer cell lines by using anti-MCM2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human Cervix Uterus using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

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  Immunohistochemical staining of paraffin-embedded 293T cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:150) (1:500)

• Immunohistochemical staining of paraffin-embedded MCF7 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded HeLa cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded A549 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded NCI-H1975 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded A375 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded HCT 116 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

• Immunohistochemical staining of paraffin-embedded HT-29 cell pellets using anti-MCM2 mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800130) (1:500)

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Specifications

Product Data

• Clone Name: UMAB238
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCM2 (NP_004517) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 101.7 kDa
• Gene Name: Homo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1, mRNA.
• Database Link:
  NP_004517
  Entrez Gene 17216 MouseEntrez Gene 312538 RatEntrez Gene 4171 Human
  UniProt ID P49736
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication