PGP9.5 (UCHL1) Mouse Monoclonal Antibody [Clone ID: UMAB244]

CAT#: UM800136CF

Carrier-free (BSA/glycerol-free) UCHL1 (PGP9.5) mouse monoclonal antibody,clone UMAB244

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and UCHL1-Knockout 293T cells (KO, Cat# LC810250) were separated by SDS-PAGE and immunoblotted with anti-UCHL1 monoclonal antibody UM800136. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• 

  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-UCHL1 (PGP9.5) Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded MCF7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded NIH/3T3 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded COS-7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded Huh7 cell pellets using anti-UCHL1 (PGP9.5) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded mouse cerebrum tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

• Immunohistochemical staining of paraffin-embedded rat cerebrum tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:150) (1:600)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-UCHL1 (PGP9.5) mouse monoclonal antibody (UM800136). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Show all 14 images

Specifications

Product Data

• Clone Name: UMAB244
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~600
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.6 kDa
• Gene Name: Homo sapiens ubiquitin C-terminal hydrolase L1 (UCHL1), mRNA.
• Database Link:
  NP_004172
  Entrez Gene 22223 MouseEntrez Gene 29545 RatEntrez Gene 7345 Human
  UniProt ID P09936
• Synonyms: HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease