FOXP3 Mouse Monoclonal Antibody [Clone ID: UMAB248]

CAT#: UM800140CF

Carrier-free (BSA/glycerol-free) FOXP3 mouse monoclonal antibody,clone UMAB248

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXP3 (RC217580, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXP3 mouse monoclonal antibody.1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

• Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200)

• Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-FOXP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800140) (1:200).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOXP3 mouse monoclonal antibody (UM800140). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

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Specifications

Product Data

• Clone Name: UMAB248
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOXP3 (NP_001107849) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.2 kDa
• Gene Name: Homo sapiens forkhead box P3 (FOXP3), transcript variant 2, mRNA.
• Database Link:
  NP_001107849
  Entrez Gene 20371 MouseEntrez Gene 317382 RatEntrez Gene 50943 Human
  UniProt ID Q9BZS1
• Synonyms: AIID; DIETER; IPEX; JM2; PIDX; XPID

Reference Data

• Protein Families: Transcription Factors