CTLA4 Mouse Monoclonal Antibody [Clone ID: UMAB249]

CAT#: UM800141CF

Carrier-free (BSA/glycerol-free) CTLA4 mouse monoclonal antibody,clone UMAB249

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue (CTLA4 negative) within the normal limits using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

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Specifications

Product Data

• Clone Name: UMAB249
• Applications: IHC
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTLA4 (NP_001032720) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.15 kDa
• Gene Name: Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.
• Database Link:
  NP_001032720
  Entrez Gene 1493 Human
  UniProt ID P16410
• Synonyms: ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway