TMEM16A (ANO1) Mouse Monoclonal Antibody [Clone ID: UMAB250]

CAT#: UM800142CF

Carrier-free (BSA/glycerol-free) ANO1 (Dog1/TMEM16A) mouse monoclonal antibody, clone UMAB250

Product Images

Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-ANO1 (Dog1) mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTAin 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800142) (1:2000)

Specifications

Product Data

• Clone Name: UMAB250
• Applications: IHC
• Recommended Dilution: IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-333 of human ANO1 (Dog1) (NP_060513) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA.
• Database Link:
  NP_060513
  Entrez Gene 101772 MouseEntrez Gene 309135 RatEntrez Gene 55107 Human
  UniProt ID Q5XXA6
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A

Reference Data

• Protein Families: Transmembrane