CD30 (TNFRSF8) Mouse Monoclonal Antibody [Clone ID: UMAB256]

CAT#: UM800146CF

Carrier-free (BSA/glycerol-free) TNFRSF8 (CD30) mouse monoclonal antibody,clone UMAB256

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8 (CD30) (RC219819, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF8 (CD30) (1:2000).

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  Western blot analysis of extracts (10ug) from K562 cell line by using anti-IDO1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-TNFRSF8 (CD30) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800146) (1:300)

• Immunohistochemical staining of paraffin-embedded Carcinoma of cell pellets (The left is K562 and the right is Jurkat) using anti-TNFRSF8 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, UM800146) (1:300).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNFRSF8 (CD30) mouse monoclonal antibody (UM800146). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

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Specifications

Product Data

• Clone Name: UMAB256
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:100~300
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 408-595 of human TNFRSF8(NP_001234) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.75 kDa
• Gene Name: Homo sapiens TNF receptor superfamily member 8 (TNFRSF8), transcript variant 1, mRNA.
• Database Link:
  NP_001234
  Entrez Gene 943 Human
  UniProt ID P28908
• Synonyms: CD30; D1S166E; Ki-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction