Tyrosinase (TYR) Mouse Monoclonal Antibody [Clone ID: UMAB257]

CAT#: UM800147

TYR (Tyrosinase) mouse monoclonal antibody,clone UMAB257

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYR (Tyrosinase) (RC221797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYR (Tyrosinase) (1:2000).

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  Western blot analysis of extracts (10ug) from SK-MEL-5 cell line by using anti-IDO1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-TYR (Tyrosinase) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 2.5 min, UM800147) (1:1200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of cell pellets (The left is SK-MEL-5 and the right is A431) using anti-TYR Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800147)n (1:1200)

Specifications

Product Data

• Clone Name: UMAB257
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:1200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-320 of human TYR (NP_000363) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.39 kDa
• Gene Name: Homo sapiens tyrosinase (TYR), mRNA.
• Database Link:
  NP_000363
  Entrez Gene 22173 MouseEntrez Gene 308800 RatEntrez Gene 7299 Human
  UniProt ID P14679
• Synonyms: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Melanogenesis, Metabolic pathways, Riboflavin metabolism, Tyrosine metabolism