CD99 Mouse Monoclonal Antibody [Clone ID: UMAB262]

CAT#: UM800152CF

Carrier-free (BSA/glycerol-free) CD99 mouse monoclonal antibody,clone UMAB262

Product Images

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800152) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800152) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800152) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800152) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CD99 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800152) (1:200)

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Specifications

Product Data

• Clone Name: UMAB262
• Applications: IHC
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.85 kDa
• Gene Name: Homo sapiens CD99 molecule (Xg blood group) (CD99), transcript variant 1, mRNA.
• Database Link:
  NP_002405
  Entrez Gene 4267 Human
  UniProt ID P14209
• Background: The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
• Synonyms: HBA71; MIC2; MIC2X; MIC2Y; MSK5X

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration