SDHB Mouse Monoclonal Antibody [Clone ID: UMAB268]

CAT#: UM800158CF

Carrier-free (BSA/glycerol-free) SDHB mouse monoclonal antibody,clone UMAB268

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB mouse monoclonal antibody.1:500

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  Western blot analysis of extracts (35ug) from 10 different cell lines by using anti-SDHB monoclonal antibody. 1:500

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800158) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800158) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800158) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800158) (1:300)

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Specifications

Product Data

• Clone Name: UMAB268
• Applications: IHC, WB
• Recommended Dilution: IHC 1:300
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SDHB (NP_002991) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.63 kDa
• Gene Name: Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.
• Database Link:
  NP_002991
  Entrez Gene 67680 MouseEntrez Gene 298596 RatEntrez Gene 6390 Human
  UniProt ID P21912
• Background: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). [UniProtKB/Swiss-Prot Function]
• Synonyms: CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHIP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease