GI24 (C10orf54) Mouse Monoclonal Antibody [Clone ID: UMAB272]

CAT#: UM800162CF

Carrier-free (BSA/glycerol-free) VISTA(C10ORF54) mouse monoclonal antibody,clone UMAB272

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VISTA (C10ORF54) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800162) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-VISTA (C10ORF54) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800162) (1:200)

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-VISTA (C10ORF54) mouse monoclonal antibody (UM800162). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:50).

Specifications

Product Data

• Clone Name: UMAB272
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:50~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C10ORF54 (NP_071436) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens V-set immunoregulatory receptor (VSIR), mRNA.
• Database Link:
  NP_071436
  Entrez Gene 64115 Human
  UniProt ID Q9H7M9
• Synonyms: B7-H5; B7H5; C10orf54; DD1alpha; Dies1; GI24; PD-1H; PP2135; SISP1; VISTA

Reference Data

• Protein Families: Transmembrane