Parathyroid Hormone (PTH) Mouse Monoclonal Antibody [Clone ID: UMAB274]

CAT#: UM800164CF

Carrier-free (BSA/glycerol-free) PTH mouse monoclonal antibody,clone UMAB274

Product Images

Immunohistochemical staining of paraffin-embedded Human gley's glands tissue within the normal limits using anti-PTH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800164) (1:4000)

Specifications

Product Data

• Clone Name: UMAB274
• Applications: IHC
• Recommended Dilution: IHC 1:4000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-115 of human PTH (NP_000306) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.86 kDa
• Gene Name: Homo sapiens parathyroid hormone (PTH), transcript variant 1, mRNA.
• Database Link:
  NP_000306
  Entrez Gene 5741 Human
  UniProt ID P01270
• Background: This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
• Synonyms: FIH1; PTH1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein