GPA33 Mouse Monoclonal Antibody [Clone ID: UMAB283]

CAT#: UM800173CF

Carrier-free (BSA/glycerol-free) GPA33 mouse monoclonal antibody, clone UMAB283

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPA33 (Cat# RC210225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPA33. (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GPA33 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800173) (1:4000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPA33 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800173) (1:4000)

Specifications

Product Data

• Clone Name: UMAB283
• Applications: IHC, WB
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GPA33 (NP_005805) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 35.6 kDa
• Gene Name: Homo sapiens glycoprotein A33 (GPA33), mRNA.
• Database Link:
  NP_005805
  Entrez Gene 10223 Human
  UniProt ID Q99795
• Background: The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008].
• Synonyms: A33

Reference Data

• Protein Families: Druggable Genome, Transmembrane