MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: UMAB286]

CAT#: UM800176CF

Carrier-free (BSA/glycerol-free) Melan A(MLANA) mouse monoclonal antibody, clone UMAB286

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Melan A (MLANA) (Cat# RC204190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Melan A (MLANA). (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-Melan A (MLANA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, UM800176) (1:1600)

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-Melan A (MLANA) mouse monoclonal antibody (UM800176). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)

Specifications

Product Data

• Clone Name: UMAB286
• Applications: IHC, WB
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 48-118 of human MLANA (NP_005502) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 13.2 kDa
• Gene Name: Homo sapiens melan-A (MLANA), mRNA.
• Database Link:
  NP_005502
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Synonyms: MART-1; MART1

Reference Data

• Protein Families: Transmembrane