PARN Mouse Monoclonal Antibody [Clone ID: UMAB143]

CAT#: UM870040

PARN mouse monoclonal antibody,clone UMAB143

Product Images

Western blot analysis of RPMI8226 cell lysate (35ug) by using anti-PARN monoclonal antibody.

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PARNmouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PARNmouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PARN mouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PARNmouse monoclonal antibody. (UM800040; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PARN mouse monoclonal antibody (UM800040). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB143
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-266 of human PARN (NP_002573) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens poly(A)-specific ribonuclease (PARN), transcript variant 1, mRNA.
• Database Link:
  NP_002573
  Entrez Gene 74108 MouseEntrez Gene 5073 Human
  UniProt ID O95453
• Background: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: DAN

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation