Tau (MAPT) Mouse Monoclonal Antibody [Clone ID: UMAB239]

CAT#: UM870131

MAPT mouse monoclonal antibody,clone UMAB239

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPT (RC216166, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPT (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

• Immunohistochemical staining of paraffin-embedded MCF7 cell pellets using anti-MAPT mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800131) (1:300)

• Immunohistochemical staining of paraffin-embedded T-47D cell pellets using anti-MAPT mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800131) (1:300)

• Immunohistochemical staining of paraffin-embedded HeLa cell pellets using anti-MAPT mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800131) (1:300)

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Specifications

Product Data

• Clone Name: UMAB239
• Applications: IHC, WB
• Recommended Dilution: IHC 1:300
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 623-758 of human MAPT(NP_058519) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.7 kDa
• Gene Name: Homo sapiens microtubule associated protein tau (MAPT), transcript variant 1, mRNA.
• Database Link:
  NP_058519
  Entrez Gene 17762 MouseEntrez Gene 29477 RatEntrez Gene 4137 Human
  UniProt ID P10636
• Synonyms: DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, MAPK signaling pathway