CPS1 Mouse Monoclonal Antibody [Clone ID: UMAB281]

CAT#: UM870171

CPS1 mouse monoclonal antibody, clone UMAB281

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Right lane) or pCMV6-ENTRY CPS1 (Cat# RC226402, Left lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPS1. (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CPS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800171) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CPS1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800171) (1:1000)

Specifications

Product Data

• Clone Name: UMAB281
• Applications: IHC, WB
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1367-1506 of human CPS1 (NP_001116105) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 165.65 kDa
• Gene Name: Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 1, mRNA.
• Database Link:
  NP_001116105
  Entrez Gene 227231 MouseEntrez Gene 497840 Rat
  UniProt ID P31327
• Background: The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.[provided by RefSeq, May 2010].
• Synonyms: CPSASE1; PHN

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism