Arginase 1 (ARG1) Mouse Monoclonal Antibody [Clone ID: UMAB288]

CAT#: UM870178

Arginase-1(ARG1) mouse monoclonal antibody, clone UMAB288

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Arginase-1(ARG1) (Cat# RC204649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Arginase-1 (ARG1). (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-Arginase-1 (ARG1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800178) (1:15000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Arginase-1(ARG1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800178) (1:15000)

Specifications

Product Data

• Clone Name: UMAB288
• Applications: IHC, WB
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARG1 (NP_000036) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 34.7 kDa
• Gene Name: Homo sapiens arginase 1 (ARG1), transcript variant 2, mRNA.
• Database Link:
  NP_000036
  Entrez Gene 11846 MouseEntrez Gene 29221 Rat
  UniProt ID P05089
• Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways