Mouse IgG2a (gamma 2a chain specific) Rat Monoclonal Antibody [Clone ID: SB84a]

CAT#: AM08017FC-N

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Product Images

Immunofluorescent Staining: Human PBMC were incubated with either mouse IgG1 anti-CD3, mouse IgG2a anti-CD5, mouse IgG2b anti- CD22, or mouse IgG3 anti-IgD. After washing, the cells were then incubated with FITC-labeled rat anti-mouse IgG1. Small lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.

Specifications

Product Data

• Clone Name: SB84a
• Applications: FC, IF
• Recommended Dilution: FLISA: 1/200-1/400.
  Flow Cytometry: ≤ 1 µg/10⁶ cells.
  Identification and enumeration of IgG2a+ cells.
  Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
  Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts with the heavy chain of IgG2a.
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.