Human IgA1 Mouse Monoclonal Antibody [Clone ID: NI 69-11 (A89-036)]

CAT#: AM20246FC-N

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), FITC

Specifications

Product Data

• Clone Name: NI 69-11 (A89-036)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA1, and Immunoblotting
  using a peroxidase labelled monoclonal antibody against FITC.
  General Recommended Dilutions:
  Histochemical Use: 1/20-1/100.
  Western blot: 1/200 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA1 isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the CH2 domain of IgA1 as tested in Haemagglutination, Haemagglutination Inhibition, Direct Binding Enzyme Immunoassay, Competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
  Cross-Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: FITC
  State: Lyophilized purified IgG fraction
  Label: Fluorescein Isothiocyanate
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG: ~3.0
• Concentration: 0.4 mg/ml
• Conjugation: FITC
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Conjugation Procedure: Conjugation is carried out using a proprietary technique for coupling to FITC, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added.