Human IgA1 Mouse Monoclonal Antibody [Clone ID: NI 69-11 (A89-036)]

CAT#: AM20246HR-N

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), HRP

Specifications

Product Data

• Clone Name: NI 69-11 (A89-036)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50
  ELISA: from 1/50 upwards
  Western blot: from 1/100 upwards
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA1 isolated from pooled Human serum
• Specificity: The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of the antiserum is restricted to a subclass specific determinant on the CH2 domain of IgA1 as tested in Haemagglutination, Haemagglutination Inhibition, Direct Binding Enzyme Immunoassay, Competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: HRP
  State: Lyophilized purified IgG fraction
  Label: Horseradish Peroxidase Enzyme marker: Horseradish Peroxidase enriched for isoenzyme C (RZ=3.2) Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
  Molar radio: Peroxidase/IgG: ~1.7
• Concentration: 0.4 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.