Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]

CAT#: AM20247AF-N

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites

Specifications

Product Data

• Clone Name: NI 512 (A89-038)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200
  ELISA: from 1/500 upwards.
  Western blot: from 1/1000 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA2 isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. 1996, J. Immunol. Methods 193, 103-148).
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: State: Ascites
  State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without foreign proteins.
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 1.0 mg/ml
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.