Human IgA2 Mouse Monoclonal Antibody [Clone ID: NI 512 (A89-038)]

CAT#: AM20247FC-N

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), FITC

Specifications

Product Data

• Clone Name: NI 512 (A89-038)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA2, and Immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
  General Recommended Dilutions:
  Histochemical Use: 1/20-1/100.
  Western blot: from 1/500 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA2 isolated from pooled Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in Haemagglutination, Haemagglutination Inhibition, Direct Binding Enzyme Immunoassay, competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. 1996, J. Immunol. Methods 193, 103-148).
  Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein Isothiocyanate isomer 1
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG ~1.3
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: FITC
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Note:

  Conjugation Procedure: Conjugation is carried out using a proprietary technique for the coupling of FITC, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added.