Human IgA2 (A2m2 Allotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 194-3 (A89-040)]

CAT#: AM20248HR-N

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), HRP

Specifications

Product Data

• Clone Name: NI 194-3 (A89-040)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50
  ELISA: from 1/60 upwards.
  Western blot: from 1/20 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA2(m)2 isolated from Human serum.
• Specificity: The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgA2(m)2, as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, latex agglutination assay and histochemistry immunoprecipitation and direct immunoperoxidase staining. (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.