Human IgA2 (A2m2 Allotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 194-3 (A89-040)]

CAT#: AM20248TC-N

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), TRITC

Specifications

Product Data

• Clone Name: NI 194-3 (A89-040)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
  General Recommended Dilutions:
  Histochemical Use: 1/20-1/100
  Western blot: from 1/200 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA2(m)2 isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to an allotype specific determinant on IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).4
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins.
  Label: TRITC
  State: Lyophilized purified Ig fraction.
  Label: Tetramethylrhodamine Isothiocyanate
  Absorption emission: 554 nm / 573 nm
  Molar radio: Fluorochrome/IgG ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: TRITC
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.