Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]

CAT#: AM20249BT-N

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Biotin


USD 680.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 69 (A89-034) and NI 184 (A89-035)
Applications ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgA isolated from pooled human serum
Specificity The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA.
It reacts with both subclasses of IgA as tested in Haemagglutination, Haemagglutination inhibition, indirect binding Enzyme Immunoassay, competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation PBS, pH 7.2 without preservatives or foreign proteins.
Label: Biotin
State: Lyophilized purified Ig fraction
Reconstitution Method Restore by adding 0.5 ml sterile distilled water.
Concentration 0.4 mg/ml
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.