Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]

CAT#: AM20249HR-N

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), HRP

Specifications

Product Data

• Clone Name: NI 69 (A89-034) and NI 184 (A89-035)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/50.
  ELISA: from 1/50 upwards.
  Western blot: from 1/100 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgA isolated from pooled human serum
• Specificity: The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay,
  immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).
  The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins.
  Label: HRP
  State: Lyophilized purified Ig fraction
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.