Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

CAT#: AM20251AF-N

Human IgE mouse monoclonal antibody, clone NI 307, Ascites

Specifications

Product Data

• Clone Name: NI 307
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting.
  General Recommended Dilutions:
  Histochemical 1/10-1/100.
  ELISA: from 1/50 upwards.
  Western blot: from 1/100 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgE isolated from Human serum
• Specificity: The reactivity of the antiserum is restricted to the IgE molecule as tested in ELISA, Immunoblotting, Immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol.
  The antiserum does not react with any other component of the
  human Ig system or any other human plasma protein as tested.
  This antiserum has not been tested for cross-reactivity with other species.
• Formulation: State: Ascites
  State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without foreign proteins.
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 1.0 mg/ml
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.