Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

CAT#: AM20251BT-N

Human IgE mouse monoclonal antibody, clone NI 307, Biotin

Specifications

Product Data

• Clone Name: NI 307
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200.
  ELISA: from 1/500 upwards.
  Western blot: from 1/1000 upwards.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified polyclonal IgE isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins.
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Molar radio: Biotin/IgG ~2.6
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.