Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

CAT#: AM20251FC-N

Human IgE mouse monoclonal antibody, clone NI 307, FITC

Specifications

Product Data

• Clone Name: NI 307
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified polyclonal IgE isolated from Human serum.
• Specificity: The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Fluorescein Isothiocyanate
  Absorption emission: 492 nm / 515 nm
  Molar radio: Fluorochrome/IgG ~3.8
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: FITC
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.