Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

CAT#: AM20251HR-N

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Specifications

Product Data

• Clone Name: NI 307
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/250.
  ELISA: from 1/250 upwards.
  Western blot: from 1/500 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified polyclonal IgE isolated from Human serum.
• Specificity: The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgE, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of
  cytoplasmic immunoglobulins.
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  Label: HRP
  State: Lyophilized purified Ig fraction
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.