Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]

CAT#: AM20252FC-N

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), FITC


USD 680.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 132 (HP 6186)
Applications ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgG1 isolated from pooled Human serum.
Specificity The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation PBS, pH 7.2 without preservatives or foreign proteins
Label: FITC
State: Lyophilized purified Ig fraction
Label: Fluorescein Isothiocyanate
Absorption emission: 492 nm / 515 nm
Molar radio: Fluorochrome/IgG ~2.7
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation FITC
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.