Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]

CAT#: AM20254BT-N

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Biotin

Specifications

Product Data

• Clone Name: NI 25-1 (HP 6207)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/250.
  ELISA: from 1/100 upwards.
  Western blot: from 1/500 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgG2 isolated from pooled Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc portion of the IgG2 molecule as tested in direct binding enzyme Immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
  Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Molar radio: Biotin/IgG ~4.2
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.