Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]

CAT#: AM20254PU-N

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Purified

Specifications

Product Data

• Clone Name: NI 25-1 (HP 6207)
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200.
  ELISA: from 1/500 upwards.
  Western blot: from 1/1000 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified monoclonal IgG2 isolated from pooled Human serum.
• Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG2 molecule as tested in Indirect Binding Enzyme Immunoassay, Immunoblotting, Immunoprecipitation and indirect Immunoperoxidase staining of cytoplasmic Ig.
  Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 0.4 mg/ml
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.