Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 25-1 (HP 6207)]

CAT#: AM20254TC-N

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), TRITC


USD 680.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 25-1 (HP 6207)
Applications ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgG2 isolated from pooled Human serum.
Specificity The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation PBS, pH 7.2 without preservatives or foreign protein
Label: TRITC
State: Lyophilized purified Ig fraction
Label: Tetramethylrhodamine Isothiocyanate
Absorption emission: 554 nm / 573 nm
Molar radio: Fluorochrome/IgG ~2.7
Concentration 0.4 mg/ml
Conjugation TRITC
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.