Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]

CAT#: AM20255BT-N

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Biotin


USD 680.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 330 (HP 6050)
Applications ELISA, IF, IP, WB
Recommended Dilution To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250. 
ELISA: from 1/1,000 upwards. 
Western blot: from 1/500 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified monoclonal IgG3 isolated from pooled Human serum.
Specificity The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 portion of the IgG3 molecule as tested in direct binding enzyme immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
Cross-Reactivity: The antibody does not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized purified IgG fraction
Molar radio: Biotin/IgG ~2.2
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation Biotin
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Note Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.